pyrroline-5-carboxylate reductase
function
osmoadaptive de novo production of proline
product
pyrroline-5-carboxylate reductase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]Gene
Coordinates
2,016,845 2,017,738
The protein
Catalyzed reaction/ biological activity
1-pyrroline-5-carboxylate + NADPH + H+ --> L-proline + NADP+ [pubmed|28824574]Protein family
[SW|pyrroline-5-carboxylate reductase family] [pubmed|28824574]Paralogous protein(s)
[protein|93000B579F630E9F0C6EED40D6661E5E15141FDE|ProI] [SW|Cofactors]
NADPH [pubmed|28824574]Effectors of protein activity
feedback-inhibited by proline [pubmed|28824574]Structure
[PDB|5BSE] (from ''Medicago truncatula'', 36% identity) [pubmed|26579138][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operons
genes
[gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]-[gene|3F47513FB71276EF1E99B8C427DC8DC0786F8C19|proJ]
description
[Pubmed|21296969]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|21784929], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulation
expressed under conditions of osmotic stress [Pubmed|21784929]view in new tabBiological materials
Mutant
MGNA-B083 (proH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1082 NBRP B. subtilis, Japan]BKE18480 ([gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGATBKK18480 ([gene|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGATReferences
Reviews
Original publications
11418582,21296969,25344233,21784929,27766092,28752945,28824574,26579138