proH [2019-08-05 08:09:49]

pyrroline-5-carboxylate reductase

Locus: BSU_18480

Isoelectric point: 7.75

Molecular weight: 31.88 kDa

Protein length: 297 aa

Gene length: 894 bp

Function: osmoadaptive de novo production of proline

Product: pyrroline-5-carboxylate reductase

Essential: no

E.C.: 1.5.1.2

Synonyms: yoxE

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of proline
• Lifestyles → Coping with stress → Coping with hyper-osmotic stress

Gene

Coordinates: 2,016,845 2,017,738

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NADPH + H⁺ --> L-proline + NADP⁺ PubMed

Protein family

• pyrroline-5-carboxylate reductase family PubMed

Paralogous protein(s)

• ProI

Cofactors

• NADPH PubMed

Effectors of protein activity

• feedback-inhibited by proline PubMed

Structure

• 5BSE (from Medicago truncatula, 36% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: proH-proJ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • expressed under conditions of osmotic stress PubMed

Biological materials

Mutant

• MGNA-B083 (proH::erm), available at the NBRP B. subtilis, Japan
• BKE18480 (proH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
• BKK18480 (proH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTCACACGTTTAAAAT, downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT

References

Reviews

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Original publications

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